2.43
1,65
1,98
0,73
1,88
1,81
2.43
2.2 Standardne tvari korištene u kalibracijskoj krivulji relativne raspodjele molekularne mase: inzulin, mikopeptidi, glicin-glicin-tirozin-arginin, glicin-glicin-glicin
3 Instrumenti i oprema
23.2
21.4
22.2
16.1
22.3
20,8
23,9
27,5
Sveukupno, udio aminokiselina u Sustarovim proizvodima je veći nego u Zinproovim proizvodima.
Dio 8 Učinci upotrebe
Utjecaji različitih izvora elemenata u tragovima na proizvodne performanse i kvalitetu jaja nesilica u kasnom razdoblju nesenja
Proizvodni proces
Ciljana tehnologija kelacije
Tehnologija emulgiranja smicanjem
Tehnologija prskanja i sušenja pod pritiskom
Tehnologija hlađenja i odvlaživanja
Napredna tehnologija kontrole okoliša
Dodatak A: Metode za određivanje relativne raspodjele molekularne mase peptida
Usvajanje standarda: GB/T 22492-2008
1 Princip ispitivanja:
Određena je visokoučinkovitom gel filtracijskom kromatografijom. To jest, korištenjem poroznog punila kao stacionarne faze, na temelju razlike u relativnoj molekularnoj masi komponenti uzorka za odvajanje, detektirane na peptidnoj vezi ultraljubičaste apsorpcijske valne duljine od 220 nm, korištenjem namjenskog softvera za obradu podataka za određivanje raspodjele relativne molekularne mase gel filtracijskom kromatografijom (tj. GPC softver), kromatogrami i njihovi podaci su obrađeni, izračunati kako bi se dobila veličina relativne molekularne mase sojinog peptida i raspon raspodjele.
2. Reagensi
Eksperimentalna voda treba zadovoljavati specifikacije sekundarne vode u GB/T6682, a reagensi, osim posebnih odredbi, moraju biti analitički čisti.
2.1 Reagensi uključuju acetonitril (kromatografski čist), trifluoroctenu kiselinu (kromatografski čistu),
2.2 Standardne tvari korištene u kalibracijskoj krivulji relativne raspodjele molekularne mase: inzulin, mikopeptidi, glicin-glicin-tirozin-arginin, glicin-glicin-glicin
3 Instrumenti i oprema
3.1 Visokoučinkoviti tekućinski kromatograf (HPLC): kromatografska radna stanica ili integrator s UV detektorom i softverom za obradu podataka GPC-a.
3.2 Jedinica za vakuumsku filtraciju i otplinjavanje mobilne faze.
3.3 Elektronička vaga: graduirana vrijednost 0,000 1 g.
4 koraka rada
4.1 Kromatografski uvjeti i eksperimenti prilagodbe sustava (referentni uvjeti)
- 4.1.1 Kromatografska kolona: TSKgelG2000swxl300 mm × 7,8 mm (unutarnji promjer) ili druge gel kolone iste vrste sa sličnim performansama prikladne za određivanje proteina i peptida.
- 4.1.2 Mobilna faza: Acetonitril + voda + trifluoroctena kiselina = 20 + 80 + 0,1.
- 4.1.3 Valna duljina detekcije: 220 nm.
- 4.1.4 Brzina protoka: 0,5 mL/min.
- 4.1.5 Vrijeme detekcije: 30 min.
- 4.1.6 Volumen ubrizgavanja uzorka: 20 μL.
- 4.1.7 Temperatura kolone: sobna temperatura.
- 4.1.8 Kako bi kromatografski sustav zadovoljio zahtjeve detekcije, uvjetovano je da pod gore navedenim kromatografskim uvjetima učinkovitost gel kromatografske kolone, tj. teorijski broj ploča (N), ne bude manja od 10000 izračunato na temelju vrhova tripeptidnog standarda (glicin-glicin-glicin).
- 4.2 Izrada standardnih krivulja relativne molekularne mase
- Gore navedene različite otopine peptidnih standarda relativne molekularne mase s masenom koncentracijom od 1 mg/mL pripremljene su usklađivanjem mobilne faze, pomiješane u određenom omjeru, a zatim filtrirane kroz organsku faznu membranu s veličinom pora od 0,2 μm do 0,5 μm i ubrizgane u uzorak, nakon čega su dobiveni kromatogrami standarda. Kalibracijske krivulje relativne molekularne mase i njihove jednadžbe dobivene su crtanjem logaritma relativne molekularne mase u odnosu na vrijeme zadržavanja ili linearnom regresijom.
4.3 Obrada uzorka
Točno odvažite 10 mg uzorka u odmjernoj tikvici od 10 mL, dodajte malo mobilne faze, ultrazvučno protresite 10 minuta, tako da se uzorak potpuno otopi i promiješa, razrijedite mobilnom fazom do vage, a zatim filtrirajte kroz membranu organske faze s veličinom pora od 0,2 μm do 0,5 μm, a filtrat analizirajte prema kromatografskim uvjetima u A.4.1.
- 5. Izračun relativne raspodjele molekularne mase
- Nakon analize otopine uzorka pripremljene u 4.3 pod kromatografskim uvjetima iz 4.1, relativna molekularna masa uzorka i njegov raspon raspodjele mogu se dobiti zamjenom kromatografskih podataka uzorka u kalibracijsku krivulju 4.2 pomoću GPC softvera za obradu podataka. Raspodjela relativnih molekularnih masa različitih peptida može se izračunati metodom normalizacije površine vrha, prema formuli: X=A/A ukupno×100
- U formuli: X - Maseni udio peptida relativne molekularne mase u ukupnom peptidu u uzorku, %;
- A - Površina vrha relativne molekularne mase peptida;
- Ukupno A - zbroj površina vrhova svakog peptida relativne molekularne mase, izračunat na jednu decimalu.
- 6 Ponovljivost
- Apsolutna razlika između dva neovisna određivanja dobivena u uvjetima ponovljivosti ne smije prelaziti 15% aritmetičke sredine dvaju određivanja.
- Dodatak B: Metode za određivanje slobodnih aminokiselina
- Usvajanje standarda: Q/320205 KAVN05-2016
- 1.2 Reagensi i materijali
- Ledena octena kiselina: analitički čista
- Perklorna kiselina: 0,0500 mol/L
- Indikator: 0,1% kristalno ljubičasti indikator (ledena octena kiselina)
- 2. Određivanje slobodnih aminokiselina
Uzorci su sušeni na 80°C tijekom 1 sata.
Uzorak stavite u suhu posudu da se prirodno ohladi na sobnu temperaturu ili na temperaturu pogodnu za korištenje.U suhu konusnu tikvicu od 250 mL odvažite približno 0,1 g uzorka (točno do 0,001 g).Brzo prijeđite na sljedeći korak kako biste spriječili da uzorak apsorbira vlagu iz okolineDodajte 25 mL ledene octene kiseline i dobro miješajte ne dulje od 5 minuta.Dodajte 2 kapi indikatora kristal violetTitrirati sa standardnom titracijskom otopinom perklorne kiseline koncentracije 0,0500 mol/L (±0,001) dok otopina ne promijeni boju iz ljubičaste u krajnju točku.
Zabilježite volumen potrošene standardne otopine.
- Istovremeno provedite slijepi test.
- 3. Izračun i rezultati
- Sadržaj slobodnih aminokiselina X u reagensu izražava se kao maseni udio (%) i izračunava se prema formuli: X = C × (V1-V0) × 0,1445/M × 100%, u formuli:
- C - Koncentracija standardne otopine perklorne kiseline u molovima po litri (mol/L)
- V1 - Volumen korišten za titraciju uzoraka standardnom otopinom perklorne kiseline, u mililitrima (mL).
- Vo - Volumen korišten za titraciju slijepe probe sa standardnom otopinom perklorne kiseline, u mililitrima (mL);
M - Masa uzorka, u gramima (g).
| 0,1445: Prosječna masa aminokiselina ekvivalentna 1,00 mL standardne otopine perklorne kiseline [c (HClO4) = 1,000 mol / L]. | 4.2.3 Standardna titracijska otopina cerijevog sulfata: koncentracija c [Ce(SO4)2] = 0,1 mol/L, pripremljena prema GB/T601. | |
| Usvajanje standarda: Q/70920556 71-2024 | 1. Princip određivanja (Fe kao primjer) | Kompleksi aminokiselina i željeza imaju vrlo nisku topljivost u bezvodnom etanolu, a slobodni metalni ioni su topljivi u bezvodnom etanolu, razlika u topljivosti između njih dvoje u bezvodnom etanolu korištena je za određivanje brzine kelacije kompleksa aminokiselina i željeza. |
| U formuli: V1 - volumen standardne otopine cerijevog sulfata utrošene za titraciju ispitivane otopine, mL; | Bezvodni etanol; ostatak je isti kao u točki 4.5.2 u GB/T 27983-2011. | 3. Koraci analize |
| Paralelno provedite dva pokusa. Izvažite 0,1 g uzorka sušenog na 103 ± 2 ℃ tijekom 1 sata, s točnošću od 0,0001 g, dodajte 100 mL bezvodnog etanola da se otopi, filtrirajte, ostatak filtriranja isperite sa 100 mL bezvodnog etanola najmanje tri puta, zatim ostatak prebacite u konusnu tikvicu od 250 mL, dodajte 10 mL otopine sumporne kiseline prema klauzuli 4.5.3 u GB/T27983-2011, a zatim izvedite sljedeće korake prema klauzuli 4.5.3 „Zagrijte da se otopi, a zatim ostavite da se ohladi“ u GB/T27983-2011. Istovremeno provedite slijepi test. | 4. Određivanje ukupnog sadržaja željeza | 4.1 Načelo određivanja isto je kao u klauzuli 4.4.1 u GB/T 21996-2008. |
4.2. Reagensi i otopine
| 4.2.1 Mješana kiselina: U 700 ml vode dodati 150 ml sumporne kiseline i 150 ml fosforne kiseline i dobro promiješati. | 4.2.2 Indikatorska otopina natrijevog difenilamin sulfonata: 5 g/L, pripremljena prema GB/T603. | 4.2.3 Standardna titracijska otopina cerijevog sulfata: koncentracija c [Ce(SO4)2] = 0,1 mol/L, pripremljena prema GB/T601. | |
| 4.3 Koraci analize | Paralelno provedite dva pokusa. Izvažite 0,1 g uzorka, s točnošću od 0,20001 g, stavite ga u konusnu tikvicu od 250 ml, dodajte 10 ml miješane kiseline, nakon otapanja dodajte 30 ml vode i 4 kapi otopine indikatora natrijevog dianilin sulfonata, a zatim provedite sljedeće korake prema točki 4.4.2 u GB/T21996-2008. Istovremeno provedite slijepi test. | 4.4 Prikaz rezultata | Ukupni sadržaj željeza X1 u kompleksima aminokiselina i željeza, izražen u %, izračunat je prema formuli (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - standardna otopina cerijevog sulfata utrošena za titraciju otopine slijepe probe, mL; | V0 - standardna otopina cerijevog sulfata utrošena za titraciju otopine slijepe probe, mL; | C - Stvarna koncentracija standardne otopine cerijevog sulfata, mol/L5. Izračun sadržaja željeza u kelatimaSadržaj željeza X2 u kelatu, izražen kao maseni udio željeza, izražen u %, izračunat je prema formuli: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| U formuli: V1 - volumen standardne otopine cerijevog sulfata utrošene za titraciju ispitivane otopine, mL; | V2 - standardna otopina cerijevog sulfata utrošena za titraciju otopine slijepe probe, mL;nom1 - Masa uzorka, g. Kao rezultat određivanja uzima se aritmetička sredina rezultata paralelnog određivanja, a apsolutna razlika rezultata paralelnog određivanja nije veća od 0,3%. | 0,05585 - masa željeza izražena u gramima, ekvivalentna 1,00 mL standardne otopine cerijevog sulfata C[Ce(SO4)2.4H20] = 1,000 mol/L.nom1 - Masa uzorka, g. Kao rezultat određivanja uzima se aritmetička sredina rezultata paralelnog određivanja, a apsolutna razlika rezultata paralelnog određivanja nije veća od 0,3%. | 6. Izračun stope kelacijeBrzina kelacije X3, vrijednost izražena u %, X3 = X2/X1 × 100Dodatak C: Metode za određivanje Zinprove stope kelacije |
Usvajanje standarda: Q/320205 KAVNO7-2016
1. Reagensi i materijali
a) Ledena octena kiselina: analitički čista; b) Perklorna kiselina: 0,0500 mol/L; c) Indikator: 0,1%-tni kristalno ljubičasti indikator (ledena octena kiselina)
2. Određivanje slobodnih aminokiselina
2.1 Uzorci su sušeni na 80°C tijekom 1 sata.
2.2 Uzorak stavite u suhu posudu da se prirodno ohladi na sobnu temperaturu ili na temperaturu pogodnu za korištenje.
2.3 U suhu konusnu tikvicu od 250 mL odvažite približno 0,1 g uzorka (točno do 0,001 g).
2.4 Brzo prijeđite na sljedeći korak kako biste spriječili upijanje vlage iz okoline u uzorak.
2.5 Dodajte 25 mL ledene octene kiseline i dobro miješajte ne dulje od 5 minuta.
2.6 Dodajte 2 kapi indikatora kristal violet.
2.7 Titrirajte sa standardnom titracijskom otopinom perklorne kiseline koncentracije 0,0500 mol/L (±0,001) dok se otopina ne promijeni iz ljubičaste u zelenu tijekom 15 sekundi bez promjene boje kao krajnje točke.
2.8 Zabilježite volumen potrošene standardne otopine.
2.9 Slijepu probu provedite istovremeno.
- 3. Izračun i rezultati
- katalonski
- Physicochemical parameters
V1 - Volumen korišten za titraciju uzoraka standardnom otopinom perklorne kiseline, u mililitrima (mL).
Vo - Volumen korišten za titraciju slijepe probe sa standardnom otopinom perklorne kiseline, u mililitrima (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adresa: Br. 147 Qingpu Road, grad Shouan, okrug Pujiang, grad Chengdu, provincija Sečuan, Kina
Telefon: 86-18880477902
Proizvodi
Anorganski minerali u tragovima
- Organski minerali u tragovima
- Svahili
- Prilagođena usluga
- Brze poveznice
Profil tvrtke
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| gudžaratski | Kliknite za upit | © Autorska prava - 2010-2025: Sva prava pridržana. | Mapa stranice NAJBOLJA PRETRAŽIVANJA Telefon |
| Tel. | 86-18880477902 | javanski | E-pošta |
| 8618880477902 | kineski | francuski | |
| Bird | kineski | francuski | njemački španjolski |
| Aquatic animals | japanski | korejski | arapski grčki |
| turski | talijanski | ||
| Ruminant animal g/head day | January 0.75 | indonezijski Afrikanerski švedski |
Polirati
- baskijski
- katalonski
- Physicochemical parameters
Hindski
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
bugarski
- Cebuanski
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- hrvatski
Nizozemski
| Application object | urdu vijetnamski | Content in full-value feed (mg/kg) | Efficacy |
| gudžaratski | Haićanin | Hausa | kinjaruandski Hmong Mađarski |
| Piglets and fattening pigs | Igbo | javanski | Kannadski Kmerski kurdski |
| Kirgizi | latinski | ||
| Bird | 300～400 | 45～60 | makedonski malajski Malajalamski |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norveški
- Paštunski
- Appearance: brownish-yellow granules
- Physicochemical parameters
Srpski
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Svahili
Tadžik
Tamil
Telugu
Tajlandski
| Application object | urdu vijetnamski | Content in full-value feed (mg/kg) | Efficacy |
| jidiš | Joruba | zulu | kinjaruandski Oriya Turkmenski |
| Ujgurski | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1,52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
